Anti-bacteria and toxicity potential of a rare Actinobacterium Pseudonocardia sp. SM1A, isolated from Mangrove Park, West Kalimantan, Indonesia

Abstract. Nofiani R, Rizky, Briliantoro Ardiningsih P. 2021. Anti-bacteria and toxicity potential of a rare Actinobacterium Pseudonocardia sp. SM1A, isolated from Mangrove Park, West Kalimantan, Indonesia. Biodiversitas 23: 453-458. This study aimed to identify evaluate antibacterial activities mangrove mud, Mempawah District, Kalimantan. The mud sample was inoculated on ISP4 agar dissolved with seawater enriched meropenem (75µg/mL) nystatin (100 µg/mL) using pour plate procedure incubated at room temperature until the appearance powdery colony. One 2 colonies successfully SM1A. SM1A morphologically biochemically identified determine its genus tested cross streak method. also cultivated shaken temperature. After 10 days, culture extracted ethyl acetate then extract well diffusion method brine shrimp lethality test (BSLT). as sp., called anti-bacteria showed active against Staphylococcus aureus, Salmonella typhi, Escherichia coli, Pseudomonas aeruginosa, Vibrio cholera, Streptococcus mutants for medium S. mutants, V. cholera ISP1. prepared ISP1 broth E. mutants. In addition, LC50=11.2570 µg/mL based BSLT categorized moderately toxic level. contained secondary metabolites having high lead compounds drug discovery.